The UA College of Medicine Quantitative Proteomics Laboratory

The Proteomics Lab

The mission of our proteomics lab is to provide collaborators the opportunity to take advantage of next generation mass spectrometers and software platforms to perform label-free quantitative proteomics.

We are here to assist collaborators in the design, execution, and analysis of quantitative proteomics studies using a collaborative and immersive approach. It is our goal to not only deliver conclusive results from quantitative proteomic projects but to also teach the principles and concepts of mass spectrometry and quantitative proteomics so that collaborators can better understand the science of the process. You do not need any prior proteomics experience - it is our responsibility to help you design, execute, and understand every step of the method. We also offer the opportunity for trainees to get hands-on bench experience in proteomic sample prep if that is something collaborators are interested in.

We have four specialties that we perform, using a “label-free” format. “Label free” means that the quantitative proteomics is performed on cells that are not labeled, so no extra steps are required to label the cells and perform the quantification. Collaborators simply lyse and go.

Interactomes
Identifying potential protein interaction partners through characterizing protein interactomes.

PTM
Identifying post-translational modifications (PTMs) and quantifying changes in PTM abundance after cell treatments (like kinase inhibitors, for example), across animal models of disease states or human control versus disease state tissue.

Proteomes
Profiling protein abundance changes across entire proteomes, from either cell culture systems, animal models, or human studies. Since we have some amazing instruments, we are able to get pretty solid depth of proteome coverage that is competitive in the current field of proteomics.

Subcelluar Fraction Proteomes
Profiling protein abundance changes across subcellular fractions, from either cell culture systems, animal models, or human studies.